79 μA μM-1 cm-2. This work provides a promising way to build more advanced nanomaterials to electrochemical detection of organic pollution based on energetically synergizing of adsorption by surface functionalization engineering.A novel magnetic C3N5 framework material (Fe3O4/C3N5) was developed as a high selectivity extractant for La(III) determination in food samples. The Fe3O4/C3N5 material was synthesized by thermal deammoniation method and has larger surface area (100.3 m2 g-1) and more effective adsorption sites compared with that of individual C3N5 material (19.4 m2 g-1). It was proved that Fe3O4/C3N5 material displayed excellent selectivity and adsorption capacity for La(III). In addition, adsorption isotherm and kinetic data indicated that La(III) adsorption based on Fe3O4/C3N5 material is a monolayer adsorption which is compatible with Langmuir model and follows a pseudo-second-order kinetic equation. By using Fe3O4/C3N5 material as extractant, an analytical method was established with low limits of detection (3σ, n = 6) of 10.4 μg L-1, reasonable recoveries ranged from 86% to 106% and good precision with the RSD less than 10.7%. The analytical method was further applied to the determination of trace La(III) in food sample. It evinced that the concentration of La(III) in sea fish is 13.2 μg kg-1 and the content of 138La is 0.138 μg kg-1, which is 1.03% of total La(III).In this paper, a cysteine-functionalized zwitterionic stationary phase (Cys-silica) was prepared based on the “thiol-ene” click chemistry between cysteine and vinyl-functionalized silica, and was further modified with bromoethane, 1-bromooctane and 1-bromooctadecane, respectively, to obtain a series of quaternary ammoniation-functionalized stationary phases (Cys-silica-Cn, n = 2, 8 and 18). These zwitterionic stationary phases were regarded as reversed-phase/ion-exchange (RP/IEC) mixed-mode chromatography (MMC) stationary phases for protein separation. The retention behaviors of proteins on these zwitterionic stationary phases were carefully investigated. The results indicated that the retentions of acidic and basic proteins on these zwitterinonic stationary phases were significantly influenced by the acetonitrile and salt concentrations, pH of mobile phase as well as the hydrophobicity of the ligand. The separation selectivity of proteins on these zwitterionic stationary phases strongly depended on the pH value of mobile phase. The baseline separation of 6 kinds of basic proteins can be achieved at pH 8.0 using Cys-silica-C2 or Cys-silica-C8 column, and 5 kinds of acidic proteins can also be separated completely at pH 4.0 with Cys-silica-C2 column. Panobinostat ic50 Moreover, owing to the quaternary ammoniation-functionalization on Cys-silica by using appropriately hydrophobic bromoalkanes, the selectivity and separation efficiency of proteins can be enhanced greatly. As a result, the acidic and basic proteins can be separated completely step by step from the complex sample by adjusting pH of mobile phase using a single Cys-silica-C2 column, which illustrates that the cysteine-functionalized zwitterionic stationary phase has a great potential for protein separation.Bioaccessibility of trace elements (Li, Be, Ti, Ga, Cu, Ag, Hg, Cd, Cs, Pt, Tl, Pb, As, Cr, Co, Ni, V, Se, Sn and Sb) and major elements (Rb, Ba, Al, Fe, Zn, Si, Ca, Mg, Mn, Mo, Sr, P and K) in tea infusions has been assessed using an in vitro dialyzability protocol. Gastric simulation (using pepsin solution) and intestinal simulation (using pancreatin and bile salts) were used to perform the in vitro digestion. ICP-MS, ICP-OES and FAES were used for elements determination in digested tea leaves, their infusions and the dialyzate fractions from tea infusions. Microwaves assisted acid digestion was used for the total element determination in tea leaves, while tea infusions were prepared by brewing tea leaves for 5 min in boiling water. The LODs for elements determined in tea leaves were in the range of 0.11-656 ng g-1 and 0.02-145.6 μg g-1 for trace and major elements, respectively. For elements’ determination in tea infusions, the LODs were ranged between 0.23 and 399.9 ng L-1 for trace elements and 0.2-1248 μg L-1 for major elements. The LODs for the elements in the dialyzable fraction varied from 0.018 to 142 μg L-1. The accuracy of the total element determination was evaluated using certified reference materials (Tea Leaves INCT-TL-1 and Rye Grass). The analytical recoveries were also assessed for analyzed elements in digested tea leaves (95-114%) and their infusions (92-115%), showing good recoveries. Among the studied elements, K was the most abundant element in tea leaves and tea infusions in almost all samples, followed by Ca, Mg, and P. Zn, Cs, and K showed the highest dialyzability percentages up to 84%, 76%, and 54%, respectively, followed by Si and Ca and K that show moderate to high dialyzability percentages. The accuracy of the dialysis process was evaluated using a mass-balance study.Recent advances in benchtop vacuum ultraviolet (VUV) spectrometers have yielded effective universal detectors for gas chromatography (GC). The ability of these detectors to acquire absorbance spectra from 125 nm to 430 nm poses an alternative to the gold standard of mass spectrometry (MS) as a sensitive and selective GC detector. The applications of GC/VUV extend into many areas. Featured here is the potential application of GC/VUV to the analysis of ignitable liquids, which may be found on debris from suspected arson fires. A particular compound class of interest is the alkylbenzenes, as they are a significant component in fuels such as gasoline, petroleum distillates, and aromatic solvents such as degreasers and cleaning solvents. To measure the sensitivity, selectivity and specificity of GC-VUV and GC-MS for alkylbenzenes we employed both library search methods and chemometric analysis using discriminant analysis. The GC-VUV detector was found to have superior specificity to the GC-MS detector in full scan mode. The GC-VUV detector was able to identify all alkylbenzenes correctly, including the correct identification of all structural isomers. LODs for both GC-VUV and GC-MS were found to be picograms on column.