Early in vitro biofilm models go back even beyond the invention of the word ‘biofilm’. In the dental field, biofilms were simply known as dental plaque and many of the first in vitro models were termed ‘artificial mouth microcosm plaques’. The purpose of this review is to highlight important elements of research from over the years regarding in vitro biofilm models, including data from our own laboratories. This helps us to interpret the models and point the way to the future development of biofilm testing. Many hypotheses regarding biofilm phenomena, particularly ecology, metabolism and physiology of volatile sulphur compounds (VSCs) and volatile organic compound (VOC) production could potentially be supported or disproved. In this way, the methods we use for screening biologically active agents including inhibitors, biocides and antimicrobial compounds in general can be improved. Hopefully, any lessons learnt in the past may be of value for the future. In this review, we focus around the need for growth rate controlled long-term biofilms; being continuously monitored using recent technical advances in bioluminescence, selective real-time electrodes, pH electrodes and continuous on-line analysis of the gas phase (both qualitatively and quantitatively). These features allow for accurate determination of growth rate and/or metabolic rate as well as pave the way towards automated assays and fine control of metabolism; impossible to achieve according to conventional biofilm theory. We also attempt to address the questions; can biofilm systems be improved to maintain long term ‘real’ or ‘true’ steady states over weeks or months, or are we limited to quasi-steady state systems for a limited period of time.The NiO films were deposited on the surface of BaTiO3 (BTO) by atomic layer deposition (ALD). The thickness of NiO film was controlled by the number of ALD cycles, which the optimum number of ALD cycles were 400 cycles. The morphology of NiO-BTO nanocomposite was observed by x-ray diffraction, scanning electron microscope, and transmission electron microscopy. The sensor based on NiO-BTO nanocomposite displays good electrocatalytic activity and high sensitivity for serotonin (at 0.36 V vs. Ag/AgCl). In the range of 0.05-5 μM, the concentrations of serotonin are linearly related to current intensity and the detection limit is 0.03 μM (S/N = 3). The NiO-BTO/GCE was successfully applied in serum samples. check details It shows that the NiO-BTO nanocomposite prepared by ALD can serve as electrochemical sensor devices and applications in the fields of biosensors.Recent advances in nanotechnology and optics have paved the way for new plasmonic devices. One of them are nanopatch antennas that are simple and, at the same time, effective devices for localizing the electromagnetic field on a scale of less than 10 nm and can be used in photonic integrated circuits as effective sources of photons, including single-photon sources. In the present study, we investigate the radiative characteristics of a submonolayer of colloidal CdSe/CdS quantum dots that form island structures in a resonator a cubic silver nanoparticle on an aluminum mirror. For detecting plasmonic nanoparticles on glass or metal surfaces, we propose a new technique involving a tunable laser and a confocal microscope. We provide a comparative study of the luminescence enhancement factors for QDs in the NPAs upon off-resonance excitation and at a wavelength close to the resonance; a significant difference in the luminescence enhancement factors (by order of magnitude) is demonstrated. A 60-fold reduction in the spontaneous emission time, as well as an increase in the radiation intensity by a factor of 330, has been obtained in the experiments. The increase in the spontaneous emission rate demonstrated for the quantum dots is explained by the Purcell effect. Full-wave simulations of electromagnetic fields were carried out for the model of the developed nanopatch antenna; luminescence enhancement factors and radiative efficiencies were calculated as well.Nanoparticles (NPs) synthesized using various chemical and physical methods are often cytotoxic which restricts their use in biomedical applications. In contrast, metallic biogenic NPs synthesized using biological systems such as plant extracts are said to be safer and their production more cost effective. NPs synthesized from plants with known medicinal properties can potentially have similar bioactivities as these plants. It has been shown that Salvia africana-lutea (SAL) and Sutherlandia frutescens (SF) have antibacterial activities. This study used water extracts of SAL and SF to produce biogenic silver NPs (AgNPs) and gold NPs (AuNPs). The antibacterial activity of AgNPs and AuNPs was tested against two pathogens (Staphylococcus epidermidis and P. aeruginosa). NP synthesis was optimized by varying the synthesis conditions which include synthesis time and temperature, plant extract concentration, silver nitrate (AgNO3) concentration and sodium tetrachloroaurate (III) dihydrate (NaAuCl4 · 2H2O) concentratiC) of the NPs and the extracts. The water extracts and SAL AuNP did not have significant antibacterial activity, while SAL AgNP and SF AgNP displayed high antibacterial activity. In conclusion, the data generated suggests that SAL and SF could be used for the efficient synthesis of antibacterial biogenic nanoparticles.Multivariate Curve Resolution with Alternating Least Squares (MCR-ALS) was for the first time successfully used to evaluate an intricate photophysical behavior, where deprotonation on the electronic ground state (S0), intra and intermolecular proton transfer processes (ESPT and ESIPT) on the electronic excited state (S1) can simultaneously be presented. In this sense, the organic dye 2-(2′-hydroxyphenyl)benzothiazole (HBT) was used as a proof-of-concept model, where MCR-ALS showed to be a powerful tool for discriminate chemical reactions that occur concomitantly on different potential energy surfaces, which include photochemical reactions. As a result, the chemometric method showed to be a straightforward approach for the determination of the acidic strengths of those equilibria were estimated as 8.61 and 1.11 to hydroxyl deprotonation on electronic ground and excited states, respectively.