• Batchelor Juel posted an update 1 year, 7 months ago

    Pharmaceutical products (PPs) are consumed worldwide and are continuously released into hydrological environments, but are not efficiently removed by sewage treatment plants. Their occurrence within the dissolved phase has been extensively studied, but only a few articles concern solid matrices. The mechanisms and extent of sorption depend on the properties of both the molecules (degradability, charge, hydrophobicity) and the matrices (clay content, organic matter content), making the spatio-temporal distribution of PPs in natural environments complex and poorly elucidated. To improve our understanding of PP distribution at a catchment scale, this study investigated different groups of molecules with varying solubility and charges, in water, suspended particulate matter, bed-load and pond sediments. The Egoutier stream, which collects the sewage effluents from two health institutions sewage effluents, is a good candidate for this investigation. Results indicate that PP occurrences in the different particulate compartments were mainly regulated by their wastewater occurrences and charges. Particulate phases all along the Egoutier stream were characterized by a limited clay content (i.e. less than 1%) and significant organic carbon content (i.e. between 0.3% and 18.0%) favouring non-specific adsorption. Therefore, neutral PPs, exhibiting higher discharge rates, persistence and hydrophobicities in comparison with cationic and anionic molecules, were the most abundant PPs in the particulate phases of this catchment. In bed-load sediments, global PP spatial distributions reflected discharge sites and sedimentary accumulation zones, mostly that of organic matter. Spatial distributions of the more hydrophobic and persistent PP in the particulate phases thus followed the stream sedimentary dynamic.Glycoside hydrolase family 1 (GH1) β-glucosidases (BGLUs) are encoded by a large number of genes and are involved in many developmental processes and stress responses in plants. Due to their importance in plant growth and development, genome-wide analyses have been conducted in the model plant species Arabidopsis thaliana, rice and maize but not in woody plant species, which have important economic and ecological value. In this study, we systematically analyzed Populus BGLUs (PtBGLUs) and demonstrated the involvement of several genes under stress conditions. Forty-four PtBGLUs were identified in Populus databases; these genes were located on 11 chromosomes, and the proteins of several PtBGLU genes were highly similar. More than 90% of PtBGLUs contain three conserved motifs. Collinearity results showed that 44 PtBGLU genes resulted from 12 tandem and 5 segmental duplication events. Phylogenetic analysis revealed that 128 BGLU genes from Populus trichocarpa, A. thaliana and Oryza sativa could be classified into 4 subgroups and subgroup Ⅱ and Ⅳ were differently having PtBGLUs and AtBGLUs. We further investigated whether several PtBGLUs responded to drought stress and ABA treatment, and the results showed that most of the selected BGLU genes were expressed in response to stress, which is consistent with previous studies involving rice and Arabidopsis homologous genes. Large numbers of stress-, hormone-, and development-related elements in the PtBGLU promoters suggest that BGLU genes may be involved in complex networks. GLPG1690 clinical trial Taken together, our results provide valuable information for an improved understanding of β-glucosidase function in woody plants.Knowledge about Solanaceae species naturally adapted to salinity is scarce, despite the fact that a considerable number of Solanaceae has been reported growing in saline environments. Lycium humile Phil. inhabits extreme saline soils in the Altiplano-Puna region (Central Andes, South America) and represents a promising experimental model to study salt tolerance in Solanaceae plants. Seeds, leaves and roots were collected from a saline environment (Salar del Diablo, Argentina). Seeds were scarified and 30 days after germination salt treatments were applied by adding NaCl salt pulses (up to 750 or 1000 mM). Different growth parameters were evaluated, and leaf spectral reflectance, endogenous phytohormone levels, antioxidant capacity, proline and elemental content, and morpho-anatomical characteristics in L. humile under salinity were analyzed both in controlled and natural conditions. The multiple salt tolerance mechanisms found in this species are mainly the accumulation of the phytohormone abscisic acid, the increase of the antioxidant capacity and proline content, together with the development of a large leaf water-storage parenchyma that allows Na+ accumulation and an efficient osmotic adjustment. Lycium humile is probably one of the most salt-tolerant Solanaceae species in the world, and, in controlled conditions, can effectively grow at high NaCl concentrations (at least, up to 750 mM NaCl) but also, in the absence of salts in the medium. Therefore, we propose that natural distribution of L. humile is more related to water availability, as a limiting factor of growth in Altiplano-Puna saline habitats, than to high salt concentrations in the soils.In the United States, all shell eggs processed under the USDA Agricultural Marketing Service voluntary grading standards must receive a shell sanitizing rinse of 100-200 ppm chlorine or its equivalent after leaving the washing process. A study was conducted to determine the concentration of peroxyacetic acid (PAA) which would be equivalent to 100-200 ppm chlorine (Cl) in reducing target organisms under the required washing conditions for shell eggs. Three isolates of Salmonella spp. (Enteritidis, Braenderup, and Typhimurium), as well as Enterobacter cloacae were used as inocula. Sanitizing treatments were negative control; deionized water; 100 and 200 ppm Cl; and 50-500 ppm PAA (7 concentrations). Considering all isolates tested, 100 and 200 ppm chlorine had 2.6 and 2.3 log cfu/mL cultural organisms remaining on shell surface; 50 and 100 ppm peracetic acid had 1.9 and 1.0 log cfu/mL cultural organisms remaining, respectively, compared with untreated control average of 3.8 log cfu/mL (P 250 ppm did not produce significant reductions in microbial populations as PAA concentration increased.

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