The work synthesized the fungal chitosan (FCS) encapsulated Gynura procumbens (GP) mediated silver nanoparticles (GP-AgNPs) for enhanced antidiabetic, antioxidant and antibacterial activity. The FCS-GP-AgNPs were characterized through UV-Visible spectroscopy, FTIR, XRD, Zeta size analyzer and TEM. The FTIR spectrum of GP-AgNPs exhibited functional groups of phenolic and flavonoids. The crystal peaks related to silver and chitosan in FCS-GP-AgNPs were demonstrated by XRD spectrum. The polydispersed nanoparticles such as AgNPs and FCS-GP-AgNPs were observed with size less then 100 nm by TEM. Zeta potential size analyzer indicated the average size and zeta potential of GP-AgNPs were 78.37 nm and -32.9 mV, whereas FCS-GP-AgNPs had 53.6 mV and 79.65 nm respectively. The FCS-GP-AgNPs was inhibited the α-glucosidase and α-amylase at 3.6 and 7.5 μg/mL respectively. Furthermore, FCS-GP-AgNPs were showed minimal inhibitory concentration (MIC) for Bacillus cereus (8.12 ± 0.12 μg/mL), Staphylococcus aureus (4.08 ± 0.47 μg/mL), Listeria monocytogenes (4.95 ± 0.32 μg/mL), Escherichia coli (8.25 ± 0.18 μg/mL), and Salmonella enterica (4.12 ± 0.64 μg/mL). In addition, the biocompatibility of FCS-GP-AgNPs was tested in A549, LN229, and NIH3T3 cells. This work concluded that FCS-GP-AgNPs proved to be biocompatible in terms of less cytotoxicity and promising in antibacterial and diabetics related enzyme inhibitory activity. V.The present study was intended to develop a papain grafted S-protected hyaluronic acid-lithocholic acid co-block (PAP-HA-ss-LCA) polymeric excipient as an amphiphilic muco permeating stabilizer for targeting breast cancer epithelial cells overexpressed with CD44 receptors. The mucopermeating, stabilizing and targeting capability of the PAP-HA-ss-LCA polymeric excipient was investigated by manufacturing tamoxifen (TMX) loaded self-nanoemulsifying drug delivery system (SNEDDS). TMX loaded PAP-HA-ss-LCA incorporated SNEDDS (TMX-PAP-HA-ss-LCA SNEDDS) were characterized for their surface chemistry, drug release, permeation enhancement, biocompatibility and antitumor activity. FTIR spectroscopic analysis showed successful synthesis of PAP-HA-ss-LCA polymer. X-ray diffraction (XRD) showed the amorphous form of TMX inside SNEDDS. The observed hydrodynamic diameter of TMX-PAP-HA-ss-LCA SNEDDS was 367.5 nm. Furthermore, Hyaluronic Acid-based Mucoadhesive Self Nanoemulsifying Drug Delivery System (SNEDDS) of TMX showed homogeneity in synthesis with low polydispersity and negative zeta potential due to stabilization with PAP-HA-ss-LCA polymer. The distinct spherical shape of the nanodroplets was evident by transmission electron microscopy (TEM). In vitro release kinetics indicated approximately >80% release within 48 h under sink conditions. Ex-vivo permeation study displayed 7.11-folds higher permeation of TMX by TMX-PAP-HA-ss-LCA in contrast to pure TMX. The biocompatibility study proved that SNEDDS formulation was safe and compatible against macrophages. In vitro cytotoxicity studies demonstrated that TMX-PAP-HA-ss-LCA SNEDDS could efficiently kill MCF-7 breast cancer cells as compared to the native TMX drug. Systemic toxicity studies proved the non-toxic nature of TMX-PAP-HA-ss-LCA in contrast to pure TMX. Based on these evidences, TMX-PAP-HA-ss-LCA SNEDDS formulation seems to be promising mucopermeating, augmented intracellular uptake with strong targeting potential for anti-proliferative activity. V.Astragalus polysaccharide (APS) is a bioactive macromolecule, which has been used to alleviate the development of Parkinson’s disease (PD), while its mechanism is still unresolved. As is generally accepted that autophagy has an important link with PD, thus it is reasonable to hypothesize that APS was involved in autophagy pathway for the presence of anti-PD. To verify this hypothesis, PD model was induced by 100 μM 6-hydroxydopamine (6-HODA) in PC12 cells and then treated with different concentration of APS. Results showed that APS could increase cell viability and the level of autophagy, improve the formation of autophagosome, promote the conversion of LC3-I to LC3-II, showing APS could improve autophagy level. Moreover, APS could down-regulate the expression of pAKT and pmTOR, and up-regulate the expression of PTEN. While these proteins are involved in PI3K/AKT/mTOR pathway, we then knocked down (KD) endogenous PI3K protein (the PI3K/AKT/mTOR pathway receptor protein) in PC12 cells. Results showed that these events regulated by APS were reversed in PI3K KD cells, shown that APS activated autophagy through PI3K/AKT/mTOR pathway for treating PD. Altogether, APS has the role of increasing autophagy, and this event was responsible for inhibiting PI3K protein to activate PI3K/AKT/mTOR pathway. Tie2 kinase inhibitor 1 chemical structure V.A biocomposite film composed of biopolymers chitosan (CS), gelatin (GE) and allantoin (AT) was fabricated by solution casting technique. The functional group interaction of the biocomposite films was inspected through the Attenuated total reflectance Fourier transform spectrometer (ATR-FTIR). The morphological changes and crystallinity of biocomposite films with varied ratios of chitosan/gelatin (CS/GE) and allantoin were examined under Scanning electron microscope (SEM) and X-ray diffractometer (XRD). The water-absorbing capacity was found enhanced by an increase in the chitosan ratio. The biocomposite films exhibit good antioxidant, anti-inflammatory properties. The biocomposite films also display enhanced stability with steady degradation under the PBS medium. The biocomposite films reveal improved antibacterial activity against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus). Blood compatibility studies explore the non-hemolytic nature. The in vitro cytotoxicity by MTT assay shows excellent biocompatibility. The fibroblast adhesion on the biocomposite film displays enhanced proliferation and viability. These significant biological properties of biocomposite film make it an appropriate candidate for wound dressing application. The supported chitosan (CS) adsorbent (FZCS) was successfully prepared by load CS on the BSCS supporter in this work. The adsorbent was characterized by elemental analysis, FT-IR, XRD, TGA, SEM and N2 adsorption-desorption techniques. The results showed the FZCS sorbent displayed the high removal rate (97.95%) for Titan Yellow (TY) anionic dye in aqueous solutions owing to its properties combining amino active functional amino group from the CS itself and higher specific surface area and mesoporous structure from the BSCS support. Isotherm data conform to the Langmuir isothermal model with a maximum adsorption capacity of 120.48 mg/g at 30 °C, suggesting monolayer adsorption of TY on the FZCS sorbent. The data were very well fitted to the pseudo-second-order kinetics. The adsorption capacity of FZCS could maintain about 70% after six cycles. The research indicated that FZCS would be a promising, eco-friendly and effective sorbent for anionic dye wastewater treatment in the near future. V.