The intracellular carbon metabolic flux pathways of denitrifying bacteria under aerobic conditions remain unclear. Here, a newly strain LSL251 was identified as Paracoccus thiophilus. Strain LSL251 removed 94.79% and 98.78% of total organic carbon and nitrate. 74.66% of nitrogen in culture system was lost as gaseous nitrogen. Moreover, 13C stable isotopic labeling and metabolic flux analyses revealed that the primary intracellular carbon metabolic pathways were the Entner-Doudoroff pathway and the tricarboxylic acid (TCA) cycle. Electrons are primarily donated as direct electron donor-NADH through the TCA cycle. Furthermore, response surface methodology modeled that the highest total nitrogen removal efficiency was 98.43%, where the optimal parameters were C/N ratio of 8.00, 32.98 °C, 50.18 rpm, and initial pH of 7.73. All together, these results have shed new lights on intracellular central carbon metabolic distribution and flux pathways of aerobic denitrifying bacteria. The aim of this work was to develop a new production, recovery and formulation process of gibberellic acid (GA3). Low-cost byproducts – citrus pulp (CP) and soybean hulls (SH) – were employed as substrate for GA3 production by Gibberella fujikuroi in semisolid fermentation. A CP/SH mixture (70%/30%) promoted high productivities both in bubble column reactor (1.66 mg L/h), and in stirred tank reactor (2.13 mg L/h). GA3 production medium cost (US$ 6.70/m3) was reduced by 85% when compared to previously reported synthetic media (US$ 44.96/m3). It was described that GA3 fermented extract has low stability, and that liquid and powder formulation of the fermented extract maintained the biomolecule activity over 6 months. Alginate and alginate/kefiran beads containing GA3 showed encapsulation efficiency of 70% and 60%, respectively. This work supports good perspectives for GA3 production using cheap substrates and simple formulation of clarified extract to favour its use in agricultural countries. Black liquor (BL) remains a critical problem during alkaline pretreatment. To solve this issue, a novel pretreatment strategy termed vacuum-assisted black liquor-recycling pretreatment, was established to pretreat sugarcane bagasse (SCB). Firstly, SCB was pretreated with 2% NaOH at 121 °C for 1 h under vacuum conditions. The produced BL was used for subsequent pretreatments after pH recovery with NaOH. The pretreated SCBs were subject to enzymatic hydrolysis and separate hydrolyzation and fermentation (SHF) without washing to neutral pH. BL was recycled on seven occasions. The results indicated that glucose yields did not significantly differ between pretreatment with NaOH and recovered BL. The enzymatic hydrolysis and the fermentation resulted in maximum 0.35 g/g of glucose yield and 116.5 g/kg of ethanol yield respectively. Compared with conventional pretreatment with NaOH, the VABLR method showed high conversion rates of cellulose into monosaccharaides, whilst preserving ~20% and ~46% of alkali and water usage, respectively. Hemicellulose hydrolysates (HH), which could be an interesting carbon source to feed mixed microbial cultures (MMC) able to accumulate high value-added compounds. This research focused on the evaluation of a culture strategy to achieve the simultaneous biological production of Levulinic Acid (LA) and Polyhydroxyalcanoates (PHA) by MMC fed with a synthetic HH (SHH). The culture strategy involves the use of sequential batch reactors (SBR) to select microorganisms capable of producing LA and PHA. This work proved that the cultivation strategy used allowed the biological production of LA, reaching 37%w/w when the SHH was composed of 85% pentoses. In addition, the simultaneous biological production of LA and PHB was possible when the SHH was enriched with acetate (45% pentoses – 50% acetate). Finally, this study showed that the composition of the SHH impacts directly on the selected microorganism genus and the type and quantity of the value-added compounds obtained. Transforming inactive phosphorus (P) to active P to recover it from waste activated sludge is important. Guanosine purchase The transformation of P fractions from high-solid sludge by the anaerobic digestion (AD) and acidification phase of AD (AAD) combined with a high temperature thermal hydrolysis process (HTTHP) was investigated. The results showed that the sequence of P release effects by three processes was HTTHP + AAD > AD + HTTHP > HTTHP + AD. The PO43–P release from high-solid sludge was directly affected by the temperature of HTTHP. At 140 °C, each process had more PO43–P release than that at 160 °C. The total amount of PO43–P release in AD + HTTHP was approximately 6 times that of HTTHP + AD. Based on the experimental results, a new process of mesophilic AD – post HTTHP was recommended, in which, enhancement of P release by sulfide ions was also proposed. The aim of this study was to explore novel source of lipase from biodiversity hot spot region of Sikkim with activity at broad temperature range for application in detergent industry. Among the isolates, Pseudomonas helmanticensis HS6 showed activity at wide range of temperatures was selected for lipase production. Statistical optimisation for enhanced production of lipase resulted in enhancement of lipase activity from 2.3 to 179.3 U/mg. Lipase was purified resulting in 18.78 fold purification, 5.58% yield and high specific activity of 3368 U/mg. The partially purified lipase was found to be active in wide range of temperature (5-80 °C) and pH (6-9), showing optimum activity at 50 °C at pH 7. Peptide sequences on mass spectrometric analysis of purified lipase showed similarity to lipase family protein of three species of Pseudomonas. Both crude and purified lipase retained residual activity of 40-80% after 3 h of incubation with commercial detergents suggesting its application in detergent industry. Current platinum-based drugs used in chemotherapy, like cisplatin and its derivatives, are greatly limited due to side-effects and drug resistance. This has inspired the search for novel platinum-based drugs that deviate from the conventional mechanism of action seen with current chemotherapeutics. This review highlights recent advances in platinum(II) and platinum(IV)-based complexes that have been developed within the past six years. The platinum compounds explored within this review are those that display a more targeted approach by incorporating ligands that act on selected cellular targets within cancer cells. This includes mitochondria, overexpressed receptors or proteins and enzymes that contribute to cancer cell proliferation. These types of platinum compounds have shown significant improvements in anticancer activity and as such, this review highlights the importance of pursuing these new designed platinum drugs for cancer therapy, with the potential of undergoing clinical trials.