-
Hobbs Calhoun posted an update 1 year, 7 months ago
The complete mitogenome of the orange-striped green sea anemone (Diadumene lineata) has been sequenced and annotated for the first time. The total length of the mitogenome is 17,552 bp with an A+T content of 62.6%. Unlike typical metazoan mitogenome, this mitogenome include 14 protein-coding genes (13 energy pathway protein coding genes, and a heg gene), two tRNAs, two rRNAs, and 19 intergenic regions. The COX1 gene possesses a homing endonuclease gene. This circular genome contains two introns, one in ND5 and another in COX1.This sequence is the first sequenced complete mitogenome in Diadumenidae and provides fundamental data for exploring complicated evolutionary relationships in Actiniaria.Epimedium brevicornu Maxim. is a traditional Chinese medicine herb with good effects on many diseases. In the present paper, the complete chloroplast genome of E. brevicornu was sequenced. The complete chloroplast genome of E. brevicornu was 159572 bp in length with 38.83% GC content, including four distinct regions large single-copy region (86535 bp), small single-copy region (17641 bp), and a pair of inverted repeat regions (27698 bp). The chloroplast genome encoded 112 unique genes, which included 78 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Phylogenetic analysis with the previously reported chloroplast genomes of Epimedium showed that E. brevicornu with small flowers at first clustered with large-flowered E. acuminatum into a strongly supported branch, but not with Epimedium species having similar floral characters.We determined the whole mtNDA genome of the Plateau brown frog (Rana kukunoris) in Helan Mountains, Inner Mongolia. The complete mitochondrial genome consists of 13 protein-coding, 22 tRNA, 2 rRNA genes, and 1 control region (CR), and its total length is 16,644 bp. Three overlaps among the 13 protein-coding genes were found ATP8/ATP6, ND4L/ND4, and ND5/ND6. The CR is 837 bp in length. The nucleotide composition is 27.49% A, 29.06% T, 14.77% G, 28.68% C. The result of phylogenetic analysis showed that there was close genetic relationship between R. kukunoris and Rana chensinensis.The Plodia interpunctella belongs to Pyralidae in Lepidoptera. The complete mitogenome of P. interpunctella was described in this study, which is typically circular duplex molecules and 15,403 bp in length, containing the standard metazoan set of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and an A + T-rich region. The gene order is the same as other lepidopterans. Except for cox1 started with CGA, all other PCGs started with the standard ATN codons. Most of the PCGs terminated with the stop codon TAA, whereas nad1 has the stop codon TAG, nad4 has the incomplete stop codon T. The phylogenetic tree showed that P. interpunctella and Corcyra cephalonica are clustered into a clade Pyralidae.In this study, the chloroplast genome sequence of Prunus sibirica was obtained from the whole genome sequencing data of Prunus sibirica. Its length is 158,248 bp, which consists of 86,331 bp large single-copy region (LSC), 26,408 bp two reverse repeat regions (IR) and 19,101 bp small single-copy region (SSC). GC content of the whole chloroplast genome is 36.71%. Those of LSC region, SSC region, and IR region were 35, 30, and 43%, respectively. There are 131 unique genes in the chloroplast genome, including 90 protein-coding genes, 33 tRNA genes, and 8 rRNA genes. A maximum-likelihood phylogenetic tree was generated from the chloroplast genomes of 10 species of Rosaceae and 11 peripheral plants. The results showed that Prunus sibirica belongs to Rosaceae and is sister to Prunus salicina.Pinus plants are the largest existing group of gymnosperms and one of the most highly differentiated taxa. Due to its huge ecological, economic, and scientific value, the genetic diversity and the relationship between the intraspecific evolution of Pinus plants have gained wide attention. In this study, the chloroplast genomes of several common pine trees in southwest and south China, including P. massoniana (masson pine), P. yunnanensis (yunnan pine), P. latteri (south asia pine), P. crassicorticea (la ya pine), and P. elliottii (slash pine), and entire cpDNA sequences were obtained. Characteristics including the structure, repeated sequence, and codon bias of the cpDNA for these five pine tree species were analyzed.The complete chloroplast genome sequence of Sargassum fusiforme is presented here. Circular mapping revealed that the complete chloroplast DNA sequence of S. fusiforme was 124,298 bp in length and had an overall AT content of 69.57%, including 137 protein-coding genes, 2 open reading frames, 28 transfer RNA genes, and 6 ribosomal RNA genes. The phylogenetic tree based on Bayesian shows that all kinds of Phaeophyceae were clustered into two monophyletic groups.The complete chloroplast (cp) genome sequence of Potaninia mongolica has been characterized in this study. The length of cp genome was 152,982 bp, containing a large single-copy region (LSC) of 84,233 bp and a small single-copy region (SSC) of 18,139 bp, which were separated by a pair of 25,305 bp inverted repeat regions (IRs). The genome contained 132 genes, including 86 protein-coding genes, 38 tRNA genes, and eight rRNA genes. The overall GC content is 37.16%. Further, phylogenetic analysis suggested that Potaninia is clustered to genus Dasiphora.In this study, we report the complete chloroplast (cp) genome of Thelypteris interrupta, a fern member, and comparative analysis with its related family members. The cp genome was 155,983 bp long, with a typical quadripartite structure including a pair of inverted repeat regions (25,614 bp) separated by a large (82,769 bp) and small (21,986 bp) single-copy (SC) region. The genome encodes a total of 88 protein-coding genes, 35 tRNA genes, and 8 rRNA genes. check details Additionally, we identified 86 RNA editing sites in 52 genes; most of the substitution was U to C (52 sites), while C to U conversion occurred in 34 positions. The phylogenetic analysis strongly supported the relationship of T. interrupta with Ampelopteris prolifera and Christella appendiculata of Thelypteridoideae family.

